Department Of Plant Pathology Research Articles

In vitro Evaluation of Botanical Extracts against Mycelial Growth of Alternaria solani

An experiment was conducted at the laboratory of the Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh. To test the efficacy of different treatments viz., Neem (Azadirachta indica), Onion (Allium cepa), Garlic (Allium sativum), Castor (Ricinus communis), Amaltas (Cassia fistula), Henna (lawsonia inermis), Madar (Calotropis gigantea) against Alternaria solani under in vitro condition. Botanicals were tested through the poisoned food technique at 5% and 10 % concentrations and 168 hours of incubation. The Minimum radial growth of observed in  Neem (Azadirachta indica) seed kernel extract @ 5% (14.8mm) followed by Henna (lawsonia inermis) leaf extract @ 10% (43.36mm), Amaltas (Cassia fistula) leaf extract @ 10% (67.13mm),  Castor (Ricinus communis) leaf extract @ 10% (67.6mm), Madar (Calotropis gigantea) leaf extract @ 10% (69.6mm), Onion (Allium cepa) extract @ 10 % (70.8mm), Garlic (Allium sativum) extract @ 10 % (72.2mm) as compared to Control (89.4mm) untreated check.

Comprehensive Disease Management Strategy for Cercospora Leaf Spot in Mungbean: Assessment of Botanicals, Fungicides, and Bio-agents In vivo

Aim: Worldwide mungbean farming is seriously threatened by the fungal disease Cercospora canescens, which causes Cercospora leaf spot and severe yield losses. This study evaluated the effectiveness of fungicides, botanicals, and bioagents in vivo with the goal of developing an efficient management approach for Cercospora leaf spot.
 Study Design: Randomized block design with ten treatments and three replications.
 Place and Duration of Study: Department of Plant Pathology, ANDUA&T Kumarganj, Ayodhya (U.P.) India located at latitude 26.47 0N, longitude 82.12 0E and altitude 113m above the sea level during kharif season, 2019.
 Methodology: The antifungal capabilities of botanical extracts were studied, the pathogen-growth-inhibiting capacity of fungicides was tested, and the potential for bio-agents to suppress the course of disease was analyzed. The effectiveness of plant extracts, including Neem leaf extract, Onion, Garlic clove extract, Tulsi, and Ginger was assessed at a concentration of 10%, against Cercospora canescens.
 Results: Ten per cent concentration was found most effective in vitro was further tested in vivo at 30 days and 45 days after sowing.  Neem leaf extract, Onion, Garlic clove extract, Tulsi, and Ginger @ 10% against Cercospora canescens
 Conclusion: It is very clear from the present studies that the plant extracts which were better in inhibiting the radial growth In vitro also showed reduction in disease incidence and per cent disease control was also higher In vivo condition.

Screening of Lentil Genotypes Against Wilt of Lentil (Lens culinaris Medik L.) Caused by Fusarium oxysporum F. SP. Lentis in Glasshouse Condition

Wilt of lentil is most damaging disease of lentil caused by the fungus Fusarium oxysporum f. sp. lentis. It causes severe crop damage from vegetative to reproducing stage which results in yield reduction. Fol isolates shows high variability and aggressiveness according to the climatic condition. So to overcome from this disease there are many ways of management like by cultural, mechanical, biological and chemical practices. Resistant variety selection is one of the best ecofriendly cultural management of the disease. So for this purpose one hundred genotypes were taken and screened through pot screening technique under glasshouse was carried out at the Student Instructional Farm (SIF), in the department of Plant Pathology, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, which is situated in the IndoGangatic plains of eastern Uttar Pradesh at a latitude of 26.470 and an altitude of 113 meters above sea level during Rabi 2020-21. One hundred genotypes of lentil were obtained from the Department of Genetics and Plant breeding, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya and IIPR, Kalyanpur, Kanpur (U.P.).Out of 100 genotypes, 41 genotypes were found highly resistant, 30 genotypes resistant, 8 genotypes moderately resistant, 10 genotypes susceptible and 11 genotypes highly susceptible to Fusarium wilt.

Screening of Germplasms for Disease Resistance Against Anthracnose of Chilli Caused by Colletotrichum capsici

Chilli (Capsicum annuum Linn.) is an important extensively grown spice crop. It belongs to the family Solanaceae. Approximately 20-27 species of chilli are found, out of which five are domesticated viz., C. annuum, C. baccatum, C. chinense, C. frutescens, and C. pubescens in different parts of the world. Chilli crop suffers from various diseases in which anthracnose caused by Colletotrichum capsici is a very serious one, causes huge yield losses in India. Severely infected fruits look straw colour and bear numerous acervuli in concentric rings. Around 27℃ temperature, 80% relative humidity and soil pH 5-6 promote infection and disease progress. The average disease incidence level ranges between 66 and 84% which results in yield loss upto 12-50% This investigation was carried out at the Main Experiment Station Vegetable Science and Department of Plant Pathology, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya (U.P) during the year 2021 -2022. Twenty genotypes of chilli were screened and none one genotypes are found resistant, nine genotypes namely, Mahycojalna, Arkalohit, Phulemukta, Arkaharita, Madhurima, Classica 152, Arkameghna, Sonakshi 44 and Divyajyoti were found moderately resistant and rest of the genotype were susceptible and highly susceptible.

Effect of Fungicides on Mycelium Growth of Rhizoctonia solani of Rice under In-vitro

Sheath blight of rice caused by Rhizoctonia solani Kuhn is emerging as very destructive disease causing heavy yield losses. This experiment was conducted at Department of plant Pathology, College of Agriculture, Rewa (M.P.) during 2018-19. This experiment was carried out in a complete randomized design (CRD) with eight treatments including untreated control. Seven fungicides viz., Flusilazole12.5%+carbendazim 25% SC, Azoxystrobin 18.2% w/w+difenoconazole 11.4 w/w SC, Azoxystrobin 11%+Tebuconazole 18.3% w/w SC, Tricyclazole 18%+ mancozeb 62%WP, Zineb 68% + hexaconazole 4% WP, Trifloxystrobin 25%+Tebuconazole 50%WG, Mancozeb 50%+carbendazim 25%WS, were evaluate against R. solani of rice at different concentrations under in-vitro by poisoned food technique. The present study, among the tested fungicides Azoxystrobin 11%+Tebuconazole 18.3% w/w SC at 200 ppm and 100 ppm was found significantly superior in inhibiting the mycelial growth of R. solani over untreated check at 96 hrs. after incubation.

Evaluation of Pigeonpea Genotypes against Sterility Mosaic Disease

Pigeonpea (Cajanus cajan L.) belonging to family Leguminosae is an important leguminous pulse crop of semi-arid tropic and subtropic regions (Asia and Africa). It is also known as red gram, arhar, tur dal. The crop is affected by several phytopathogens, of which, pigeonpea sterility mosaic disease/virus, and transmissible by eriophyid mites (Aceria cajani) has been a major bottleneck. Yield losses up to 95 per cent or even 100 per cent in severe Sterility Mosaic Disease incidence were reported. Therefore, present investigation on screening of pigeon pea genotypes against the sterility mosaic disease was carried out during Kharif, 2018 at the Department of Plant Pathology, College of Agriculture, Latur. In present study, about 27 entries of pigeonpea were screened under natural epiphytotic against pigeonpea sterility mosaic disease. Of these GRG-152, ICP-2376, BRG-5, BRG-4, ICPL-15048, BSMR853, BSMR-736, BRG-1and BRG-3 were resistant; MPV-106, RVSA-16-1, IPA-16-8 and BRG-2 were moderately resistant, whereas, PUSA-2017-01, TDRG-58, ICP-8863, PUSA-2018, PUSA-2018-1, PUSA-2018-2, PUSA-2018-3 ,PUSA-2018-5, AKTE-12-04, KRG-244, PADT-16, RKPV-912, JKM-189 and TJT-501 were susceptible to Sterility Mosaic disease.

In vitro Antifungal Efficacy of Silver Nanoparticles against Fusarium oxysporum f. sp. Lycopersici in Tomato

Present investigation on Mycosynthesis of silver nanoparticles and antifungal activity of silver nanoparticles against Fusarium oxysporum f. sp. lycopersici in tomato was carried out at department of Plant Pathology, College of Agriculture, Latur to find out antifungal activity of silver nanoparticles against Fusarium oxysporum f. sp. lycopersici in tomato. Mycosynthesis of silver nanoparticles produced from the fungus Trichoderma harzianum. Characterization of silver nanoparticles were carried out by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM) which revealed that synthesized nanoparticles were having the UV absorption peak at 420 nm and nanoprticle size was 50 nm. Silver nanoparticles demonstrated significant antifungal activity against Fusarium oxysporum f. sp. lycopersici in tomato by using Agar well diffusion method and Poisoned food technique. In Agar well diffusion method, the highest zone of inhibition 18.66 mm was recorded at 100 ppm concentration than other treatments. In poisoned food technique, the suspension of silver nanoparticles at 100 ppm concentration recorded highest (75.19%) inhibition. This was followed by 50 ppm, 30 ppm, 10 ppm conc. and Trichoderma culture filtrate which recorded 66.67%, 58.89%, 54.45% and 51.45% inhibition, respectively. Growth of the Fusarium oxysporum f. sp. lycopersici in tomato decreased drastically with increase in the concentration of the silver nanoparticles.

Effect of Selected Leaf Extracts on the Mycelial Radial Growth of Phytophthora sp. Causing Corm Rot of Colocasia (Colocasia esculenta L.)

In the present study the pathogenicity of Phytophthora sp. causing corm rot of colocasia was proved by Koch’s postulates. Sixteen infected corm samples were collected from different markets in two districts (West Godavari and East Godavari) of Andhra Pradesh and pot experiment was carried out in the Department of Plant Pathology, SHUATS, Prayagraj during Rabi season (2022-23).  Out of those, the sample which collected from Kovvur Rythu Bazar, West Godavari district in Andhra Pradesh shows high pathogenicity. Five leaf extracts viz., neem, mint, lantana, tulsi and periwinkle @ 5, 10 and 15% concentrations were evaluated by using poisoned food technique against Phytophthora sp. Among all the leaf extracts, neem leaf extract @ 5, 10 and 15% concentrations recorded minimum radial growth (26.43, 24.73 and 22.77 mm, respectively) and maximum per cent inhibition (33.27, 37.59 and 42.55%, respectively) of Phytophthora sp. at 120 hrs of incubation as compared to other leaf extracts, treated (Metalaxyl) and untreated checks. The present study is limited to one trial in Prayagraj conditions, therefore to substantiate the present results more such trials are required in future for further recommendation.

Marasmius vagus (the Wandering Creamsicle), One of Florida’s Most Common Lawn Mushrooms

Marasmius vagus is one of the most common lawn mushrooms in the state of Florida. The purpose of this publication is to introduce Floridians to this mushroom, provide basic information about its biology, and aid in identification. Written by Sarah Prentice and Matthew E. Smith, and published by the UF/IFAS Plant Pathology Department, February 2024.

Effect of Bacillus subtilis, Soil Amendments and Microalgae Treatment on Fusarium equiseti of Turmeric (Curcuma longa L.) Prayagraj, India

Rhizome rot of turmeric caused by Fusarium equiseti emerge as a significant soil-borne threat to crop health. An experiment was conducted to evaluate the effect of Bacillus subtilis, soil amendments and microalgae treatment on Fusarium equiseti of Turmeric (Curcuma longa L). Experiment was conducted under field condition at the courtyard of department of Plant Pathology, SHUATS, Prayagraj, during kharif season of 2022. Various soil treatments including farm yard manure (FYM), spent mushroom compost (SMC), and microalgae were used, along with rhizome treatment utilizing Bacillus subtilis. The experiment was carried out in Randomized Block design (RBD) with three (3) replications. Results revealed that in the soil application, (T5) i.e., the combination of all the treatments recorded minimum disease intensity (59.26%) which was significant over other treatments and control (T0) (33.86%). To evaluate the radial growth (mm) of Fusarium equiseti in turmeric, seven botanicals from Manipur viz., Zanthoxylum acanthopodium, Phlogacanthus thyrsiformis, Solanum xanthocarpum, Centella asiatica, Drymaria cordata, Persicaria chinensis and Gynura cusimbua were investigated in vitro at 10% and 30% concentration. The antagonistic effects of various botanicals were evaluated in vitro, demonstrating inhibition of mycelial growth. Among the tested botanicals, Zanthoxylum acanthopodium inhibited the highest inhibition rate (86.27%), followed by Phlogacanthus thyrsiformis (77.45%), Solanum xanthocarpum (75.48%), Persicaria chinensis (68.62%), Drymaria cordata (65.68%), Gynura cusimbua (62.74%) with Centella asiatica displaying the lowest inhibition rate (57.84%), all at a concentration of 30%. Overall, our findings highlight the potential of integrated management strategies involving biological control agents and soil amendments for sustainable management of Fusarium equiseti in turmeric cultivation. Implementation of these strategies holds promise for improving crop health, productivity, and sustainability in turmeric production systems.

Prospecting the Effectiveness of Fungicides and Bioagents against Colletotrichum gloeosporioides (Penz.) Penz & Sacc.

Aims: The leaf spot caused by Colletotrichum gloeosporioides (Penz.) Penz & Sacc. is one of the most serious disease in all mango growing regions of the world. In vitro bioassay of fungicides and bioagents were performed to evaluate the effectiveness of different fungicides and bioagents against C. gloeosporioides.
 Study Design: Poison food techique for fungicides bioassay and dual culture technique for bioagents bioassay.
 Place and Duration of Study: The laboratory studies were conducted in the Department of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari, Gujarat, India during 2020–2021.
 Methodology: The poison food technique assessed the efficacy of systemic, contact and combination fungicides against C. gloeosporioides. While the dual culture method was employed to gauge the potency of biocontrol agents, against C. gloeosporioides. 
 Results: In laboratory screening, systemic fungicides such as carbendazim and difenconazole were shown to have 100% growth suppression at 500 ppm. Mancozeb and copper oxychloride, two contact fungicides, demonstrated 100% growth inhibition of C. gloeosporioides at 2500 ppm . The combi-product fungicides tricyclazole 18% + mancozeb 62%, hexaconazole 4% + zineb 68%, and carbendazim 12% + mancozeb 63% were shown to exhibit 100% growth inhibition at 2000 ppm. Using the dual culture method, five distinct bioagents were tested in vitro for their ability to prevent the development of C. gleosporiodes. Trichoderma harzianum showed up as one of them to be a powerful and effective antagonist of C. gleosporiodes.
 Conclusion: In vitro fungicide and bioagent testing provides preliminary data on the effectiveness of fungicides against pathogens, guiding field testing. The study evaluated the effectiveness of fungicides and bioagents in suppressing the leaf spot pathogen of mango.

Bioconsortia: A Potential Ecofriendly Bio-Based Product to Enhance the Productivity of the Green Gram

The present investigations on the studies to see the effect of seed biopriming with bacterial consortia in green gram were conducted in a field experiment employing randomized block design with nine treatments and three replications during kharif season 2022-2023 at the Post Graduate Institute, research farm of the Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar, Maharashtra. The result revealed that, among the treatment, T4 recorded the highest average plant height (65.66 cm) and average number of leaves (22.79 plant-1), average number of branches (5.23), average number of pods (30.93), average grain yield (9.18 q ha-1), average stalk yield (24.25 q ha-1) and 1000 seed weight i.e. test weight (33.78 g).

Effects of Bio-inoculation on the Growth of Cabbage as Well as the Disease Progression of Alternaria Leaf Spot

Cabbage is one of the common Cole crop which is widely grown throughout India. It is affected various disease including, Alternaria leaf spot, black rot, club root, downy mildew and powdery mildew. Alternaria leaf spot, caused by Alternaria species such as A. brassicae and A. brassicicola, has been documented on every continent and is a prevalent disease affecting cabbage. Among the various diseases impacting cabbage, it stands out as a major contributor to yield loss, averaging between 32-57%. An experiment Effects of bio-inoculation on the growth of cabbage seedlings, as well as the disease progression of Alternaria leaf spot under challenge inoculation was conducted at Department of Plant Pathology, Rama University, Kanpur during 2023. Result of the study revealed that. application of Trichoderma as a seed treatment led to higher root and shoot dry biomass (0.080 and 0.021 mg/ seedling respectively), higher % increase in  root and shoot dry weight (61.25and 82.78%) was found in T1 (Seed treatment with  trichoderma  harzianum) whereas highest  root: shoot ratio (0.222) was  reported in treatment T2.

Isolation, Purification and Identification of Xanthomonas oryzae pv. oryzae

The present study was conducted during kharif (July-November, 2020) at the Department of plant pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari, Gujarat, India to  isolate, purify and identify the pathogen responsible for rice bacterial blight. The pathogen was isolated from infected rice leaves and purified through streaking techniques, with its cultural and morphological characteristics utilized for identification. The analysis revealed that colonies grown on nutrient agar media exhibited traits consistent with Xanthomonas oryzae pv. oryzae, appearing raised, creamy yellow, smooth, entire and buttery as cultural  characteristics and the bacterium as possessing a rod-shaped structure, gram-negative staining, encapsulation and non-spore formation as morphological characteristics. Furthermore, a pathogenicity test was conducted on the susceptible rice cultivar GR-11, cultivated in pots under controlled conditions within a net house was successfully satisfying Koch’s postulates. Rice (Oryza sativa L.) is one of the important cereal crop and a crucial dietary staple grown extensively in Gujarat, where it plays a significant role in ensuring food security and sustainability. However, rice bacterial blight remains a persistent threat, leading to substantial losses in both quantity and quality. To address this issue the investigation provided valuable insights into understanding the pathogen, thereby contributing to the development of cost-effective solutions for managing rice bacterial blight, which helps in increasing crop yield to the rice growing farmers of Gujarat.

Prevalence and Epidemiology of Apple Powdery Mildew in Northwest Himalayan Region of Himachal Pradesh

A survey was conducted to assess the incidence and severity of powdery mildew of apples during April to July of 2020 and 2021 at the different locations of Kinnaur district, Himachal Pradesh, India. Assessment scale of Hofer et al. (2019) with slight modification was used for cultivar screening. Apple seedlings were grown in the pots in the polyhouse of the Department of Plant Pathology in order to test the pathogenicity of Podosphaera leucotricha. Pathogenicity was confirmed through inoculation tests by gently pressing diseased leaves on young leaves of five asymptomatic, potted one-year-old Gale Gala seedlings of apple. Five non-inoculated plants were used for a control treatment. Overall, twenty different apple cultivars were screened against powdery mildew planted under field conditions at Krishi Vigyan Kendra Sharbo, Kinnaur, Himachal Pradesh, India. Out of these cultivars, five cultivars viz. Red Delicious, Royal Delicious, Red Velox, Auvil Early Fuji and Oregon Spur II, were found to be less susceptible to Podosphaera leucotricha, causing powdery mildew of apples, while Granny Smith, Golden Delicious, and Gale Gala were found extremely susceptible. Gala series of cultivars were found to be extremely susceptible to the pathogen. Incidence of the disease ranged from 25.42% to 53.80% and severity from 39.75% to 66.25%, respectively. According to the present study the disease initiated during the first week of May and peaked in the month of July in district Kinnaur. Temperatures between 18–24°C and RH between 50–65% were found to be favourable for the disease development. The conditions in the region were found to favourable for disease development and spread.

Effect of Antagonists and Botanicals Against Xanthomonas oryzae pv. oryzae In Vitro

The present study was conducted during kharif (July-November, 2020) at the Department of plant pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari, Gujarat (396 450), India to examine the efficacy of four recognized antagonists and a control against Xanthomonas oryzae pv. oryzae. By employing paper disc method the results indicated that Bacillus subtilis exhibited the highest radius of inhibition. Following this, Pseudomonas fluorescens, Trichoderma harzianum and Trichoderma viride were identified as the next most effective antagonists. In addition, ten different botanical extracts, including a control, were examined for their inhibitory impact on the bacterial growth of bacterial blight at a 5% concentration using the poisoned food technique. Among these extracts, garlic clove extract demonstrated the maximum inhibition radius against the pathogen, followed by tulsi leaf extract and neem leaf extract, attributed to potent toxic principles. Conversely, extracts from karanj, nilgiri, dhatura, jetropha and rhizomes of ginger, turmeric and onion bulb were found to be less effective against Xanthomonas oryzae pv. oryzae. The goal of this study provided valuable insights to determine the potential antagonists and botanicals against bacterial blight of rice in vitro with experimental design of Completely Randomized Design which offer a cost effective solution for the disease, in increasing crop yield to the rice growing farmers of Gujarat without disturbing ecosystem and without creating the problems of pollution.

In vitro Inhibition of Rhizoctonia solani Radial Growth by Native Mycoflora: Implications for Root Rot Disease in Chili

In the course of the study, native bioagents isolated from the rhizosphere of the chili crop were used to manage the soil-borne pathogens Rhizoctonia solani. The study was conducted in the laboratory of the Department of Plant Pathology and Nematology, RPCAU, Pusa, Bihar in the year 2020-23. The soil microflora (fungal and bacterial) was isolated from the rhizosphere of Chili and screened in vitro by evaluating their antagonistic potential against Rhizoctonia solani, and resultantly two fungal and two bacterial isolates were found most effective in inhibiting the mycelial growth of the pathogen over control. The maximum percent inhibition was recorded in the case of Trichoderma harzianum (71.98%) followed by Trichoderma viride (62.54%) and among the bacterial isolates maximum inhibition was recorded in the case of RB1 that inhibit (69.38%), followed by RB6 (66.42%). Overall, these findings suggest that the combination of Trichoderma and Bacteria could be an effective and sustainable method for reducing the radial growth of Rhizoctonia solani causing Root rot disease in chilli. Rhizoctonia solani was established as a causal organism of chili. The use of Bio-control agents is an eco-friendly approach and a good option to manage soil borne phyto-pathogens. These biological control agents either use the mechanism of antibiosis or mycoparasitism against the fungal pathogen. Evaluation of Trichoderma spp. and Bacterial isolates against Rhizoctonia solani showed that significantly reduced the mycelial growth of Rhizoctonia solani in vitro.

Influence of Different Culture Media on Growth and Sporulation of Colletotrichum capsici

Aim: To know the best culture media for growth and development of Colletotrichum capsici causing chilli anthracnose.
 Study Design: Twelve different culture media were selected and three replications were maintained.
 Place and Duration of Study: Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnatka, which was carried out in the year 2021-2023.
 Methodology: The laboratory study was conducted to know the best nutrient media for the growth and sporulation of the pathogen. The pathogen was isolated from infected chilli fruit using tissue isolation technique and pure culture was maintained. Twelve different nutrient media were selected and tested for the growth and sporulation of the pathogen in three replications.
 Results: Among twelve media tested, Red chilli dextrose agar have promoted the maximum growth of C. capsici (90.0 mm), which was followed by carrot dextrose agar and potato carrot agar (89.50 mm). Green chilli extract agar significantly promoted minimum growth of C. capsici (33.50 mm), which was followed by red chilli extract agar (48.00 mm) and Sarbouraud’s agar (48.50 mm). The cultural characters were varied among the media.
 Conclusion: Potato dextrose agar and carrot dextrose agar media were proved to be best media for growth and sporulation of Colletotrichum capsica.

Potential of Native Microflora of Banana, Chili and Garlic Intercropping System for Bio-control of Rhizoctonia solani Causing Root Rot of Chili

Root rot disease caused by Rhizoctonia solani is a serious problem in chili pepper production, leading to significant crop loss and reduced yield. The chemical control method for controlling this soil borne disease is not preferred due environmental concerns and harmful effect on beneficial soil microflora. Therefore, there is an increasing demand for safer and sustainable approaches. In the present investigation which was conducted in laboratory of the Department of Plant Pathology and Nematology, RPCAU, Pusa, Bihar during the year 2021-23, the microflora isolated from the rhizosphere of Banana+Chili+Garlic intercropping system  were evaluated for their effectiveness against Rhizoctonia solani causing  root rot in Chili. In course of study the isolates of Trichoderma and Bacillus significantly reduced the radial growth of Rhizoctonia solani. The maximum growth inhibition was recorded by the Trichoderma isolate - GITr5 causing 71.40 % inhibition of the pathogen growth. This was followed by CITr4 with 67.41% and BITr1 with 62.86 %inhibition of the pathogen. Among the bacterial isolates, The isolate CIRB1 was found to inhibit the target pathogen by 71.22% followed by BIRB8 causing 66.92% inhibition of the pathogen.

The Pathogenicity Test, Cultural and Morphological Characterization of Anthracnose (Colletotrichum lindemuthianum) of Green Gram (Vigna radiata L. Wilczek)

Aims: The objective of this study was to identify the appropriate anthracnose fungus of green gram using symptomatology, identification, pathogenicity test and characterization of samples.
 Study Design:  Pathogenicity test, cultural and morphological characterization.
 Place and Duration of Study: The laboratory studies were conducted in the Department of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University, Navsari, Gujarat during rabi 2020-21.
 Methodology: The sample was collected and subjected to isolation, purification and identification of pathogen. Four different pathogenecity tests were conducted to verify the pathogen causing anthracnose of green gram. On the other hand, the cultural and morphological characteristics of pathogen were studied. 
 Results: The pathogenecity test confirmed that the pathogen as Colletotrichum lindemuthianum in all four tests. Spraying inoculum after needle pricking and carborandum powder producing 100% infection in inoculated plants while 96% in spraying spore suspension without any abrasion. The less per cent of infection was observed in seed inoculated plants. The cultural studies of C. lindemuthianium shown sub-aerial whitish-to-pink cottony growth and pinkish-to-brown pigmentation under the culture plate. The morphological study of C. lindemuthianium observed as single-celled, hyaline, cylindrical, and smooth with oil globules. The acervuli were blackish brown and measure 181.3 × 254.6 µm. The setae were visible through the conidial mass with 1-3 septations.
 Conclusion: A study on green gram anthracnose confirmed C. lindemuthianum as pathogen in all four pathogenicity test with needle pricking and carborandum powder producing 100% infection in inoculated plants while 96% in spraying spore suspension without any abrasion. Cultural studies revealed whitish-pink cottony growth and pinkish-brown pigmentation. C. lindemuthianium was morphologically observed as single-celled, hyaline, cylindrical, smooth, with oil globules, blackish brown acervuli, and visible setae through conidial mass with 1-3 septations. Therefore, these findings are important for identifying the anthracnose disease of green gram.